Analysis of the genes encoding the MBL-fold metallohydrolase superfamily proteins of the Pseudomonas putida BS3701 petroleum component-degrading strain

Objective. To determine whether the genes whose products are annotated as «MBL-fold metallohydrolase superfamily» related to proteins of metallo-β-lactamase group. Materials and Methods. Sequences 7 were analyzed using following resources: ClustalW, IQ-TREE, iTOL. Selection oligonucleotides for real-time PCR was performed Primer-BLAST resource. The level gene expression assessed PCR. MIC MBC measuring cefepime meropenem. double-disc method with EDTA used presence MBL in strain. Results. Analysis nucleotide sequences studied revealed that all them not included clade containing metallo-β-lactamase. In exponential growth phase, mRNAs corresponding found. Determination a low resistance antibiotics β-lactamase phenotypic test negative P. putida strain BS3701. Conclusions. investigated metallo-β-lactamases. They involved BS3701